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The virulence of Actinobacillus pleuropneumoniae strains isolated from different outbreaks of porcine pleuropneumonia is not uniform. In order to study if this variability would disappear during repeated passage, a virulent strain (Ajax) was passaged 15 times at 37 degrees C in various media. After each passage, a sufficient number of colonies were taken to be able to inoculate a piglet. All animals died after 15 passages without any of the well-adapted strains showing any reduction in the ability to cause typical pulmonary lesions. In contrast, one of the poorly adapted strains from a previous passage (Kartha) still showed as virulent as its predecessor (passage 5). The other poorly adapted strain (Kootstra) only slightly attenuated (less than 1 log unit) its virulence. The effect of cold adaptation on the virulence of A. pleuropneumoniae was studied by passage of

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